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yisK [2018-09-13 17:49:40]
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yisK [2018-09-13 17:49:40]

modulator of Mbl activity, similar to 5-oxo-1,2,5-tricarboxilic-3-penten acid decarboxylase
locus
BSU10750
pI
5.45
mw
32.99 kDa
protein length
301 aa Sequence Blast
gene length
903 bp Sequence Blast
function
control of cell division
product
modulator of Mbl activity
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    1,152,244 → 1,153,149

    Phenotypes of a mutant

  • overexpression results in loss of cell width and cell lysis (suppressed by mutations in [protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|Mbl] that affect the ATP-binding site) [Pubmed|27215790]

    • The protein

    Structure

  • [PDB|3R60] (from ''Mycobacterium Abscessus'', 42% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|07E876715DCF551B8BDB6AA5D33C700696B4E730|yisK]-[gene|BDA8B49AC4AE81383D84337C7FEE9D88EA92AED3|yisL]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|27215790], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|27215790], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
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    Biological materials

    Mutant

  • MGNA-B291 (yisK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1290 NBRP B. subtilis, Japan]
  • BKE10750 (Δ[gene|07E876715DCF551B8BDB6AA5D33C700696B4E730|yisK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATTCCTCCATCATC, downstream forward: _UP4_TGAATAAAACTGGAGGGCGG
  • BKK10750 (Δ[gene|07E876715DCF551B8BDB6AA5D33C700696B4E730|yisK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTATTCCTCCATCATC, downstream forward: _UP4_TGAATAAAACTGGAGGGCGG

    • References

  • 27215790